Laboratory Technician Cell Biology

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/7c8r6g

The research group of Precision Medicine led by Prof. Salvatore Piscuoglio ((email hidden)) will be based al the Humanitas University, Milan, Italy, from July 2024 is seeking one highly motivated Laboratory Technicians in Cell Biology (100%) to join our team of bioinformaticians, molecular biologists, pathologists, and clinical fellows.

The candidates will develop, optimize and apply cutting-edge cellular/ molecular methods to generate 3D in vitro and ex vivo tumor models that adequately recapitulate the tumor architecture and mimic the cell-to-cell interactions within the tissue microenvironment. The specific tasks will include the generation and maintenance of patient-derived tumor organoids, patient-derived tumor fragments, fibroblasts, endothelial cells, Peripheral Blood Mononuclear Cells (PBMCs), and Tumor Infiltrating Lymphocytes (TILs).

Main responsibilities include

processing patient-derived samples according to pre-established guidelines (e.g., tissue dissociation, isolation of PBMCs/ TILs, endothelial cells, sample freezing and storage,



nucleic acids and protein extraction, and patient-derived tumor organoid/ fragment generation and maintenance)
generation and maintenance of cell cultures and execution of experiments on cell cultures
updating the database of patient samples and reagents
documenting the experiments
providing general support for lab maintenance within the scope of this project

Profile

"Laboratory Technician" degree or a Bachelor/Master degree in molecular biology, biotechnology or a related discipline
excellent technical expertise in molecular/ cell biology techniques
hands-on experience handling and processing patient samples
hands-on experience with cell culture and flow cytometry techniques is a must
experience in primary cell culture and 3D cultures is highly desirable
technical expertise in organoid generation and maintenance is a plus
once trained, the ability to work independently
organized and detail-oriented; a

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/7c8r6g