Postdoc Calviello Lab - Ciliopathies Research Programme

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/7gwlp3

ppbBuild the science that shapes the future of human health. /bbr/bApplication closing date: /b /ppHuman Technopole (Milan) is a rapidly expanding life science institute where international researchers and cutting-edge technologies converge to accelerate biomedical discovery. Our mission is to transform bold scientific ideas into advances that improve human health. /ppbAbout the role: /b /ppThe position will be a joint appointment between the Functional Genomics and the Molecular Cell Biology Research Centres, and jointly supervised by Dr. Lorenzo Calviello and Dr. Gaia Pigino. The position is financially supported by the Human Technopole Ciliopathies Flagship Research Programme ,a multidisciplinary initiative that brings together several Research Groups to dissect the molecular mechanisms underlying ciliary function at multiple scales. Our overarching goal is to achieve a mechanistic understanding of ciliopathies, a diverse group of human disorders characterized by heterogeneous clinical manifestations that ultimately arise from dysfunction of the ciliary organelle. /ppThe successful candidate will be work in a world-class environment with international leaders in mRNA biology, Structural Biology, Cell Biology, advanced analytics and cutting-edge -omics technologies, and apply state-of-the art methods in transcriptomics, biochemistry, fluorescence and cryo-electron tomography to illuminate the biology of RNA and protein synthesis in subcellular space. /ppThis project investigates how RNA localization and compartment-specific translation contribute to the assembly and maintenance of the primary cilium, addressing a largely unexplored layer of organelle regulation. By integrating subcellular transcriptomics, selective ribosome profiling, proteomics, and structural approaches, we aim to define the ciliary transcriptome, identify localized translation events,



and characterize ribonucleoprotein complexes that drive ciliogenesis. /ppCollaborations and interactions with all the involved Research Groups are highly encouraged to develop independent research projects and prepare for a successful future in interdisciplinary science. /ppbWhat you’ll bring: /b /ppEssential /pulliPhD degree in molecular biology or related discipline, with a focus on RNA biology, biochemistry, structural biology and/or cell biology. /liliProven track record of research activity in molecular biology. /liliStrong background in biochemistry, mRNA translation and cell biology. /liliIndependence in performing standard mammalian tissue culture and basic molecular biology and microscopy tasks. /liliAbility to optimize conditions for RNA, proteins, and protein-RNA complex extraction and purification. /liliFluency in English /li /ulpPreferred /pulliExpertise in Protein/RNA biology in subcellular compartments. /liliExperience in optimizing experimental conditions for isolating RNP complexes (e.g. ultracentrifugation, polysome profiling, immunoprecipitation, proximity labelling, chromatography methods). /liliPrevious experience with cilia biology. /liliFamiliarity with cryo-EM and/or cryo-electron tomography. /liliIndependence in transcriptomics/proteomics data analysis and interpretation. /liliExperience with the study of local translation. /li /ulpOrganizational and social skills /pulliAbility to work independently. /liliWillingness to frequently discuss results and ideas, highlighting controls and potential pitfalls,



using concise and clear presentations. /liliMotivation to interact lively with colleagues in the labs involved in the collaboration, other departments, and the larger scientific community in various events (journal clubs, lab meetings, seminars, international conferences, etc.). /liliStrong communication skills and respect for their own and other colleagues’ work, towards fostering a transparent, collaborative, stimulating, and welcoming scientific environment. /liliWillingness in identifying third-party funding sources (this is not a strict requirement). /li /ulpbWhy Human Technopole /bbr/HT offers an international and dynamic workplace, competitive welfare provisions, flexible working policies and relocation support. Researchers moving to Italy may vantaggi from attractive tax benefits. We promote work–life balance and provide parental support initiatives. /ppbHow to apply /b /ppInterested candidates should submit: /pulliCurriculum Vitae /liliCover Letter detailing relevant experience, motivation and list of publications /liliContact details of at least three referees /li /ulpThis is a 4-year contract offered under CCNL Chimico Farmaceutico, Level B2 /ppSalary range: € 40.000,00 - € 43.000,00 depending on candidate's seniority /ppThe position is based in Milan, Italy, within our vibrant international campus. /ppbWe strongly encourage applications from candidates belonging to protected categories (L. 68/99). /b /ppThe Foundation reserves the right, at its sole discretion, to extend, suspend, modify, revoke, or cancel this job posting without giving rise to any rights or claims whatsoever in favor of the candidates; the Foundation reserves, however, the right not to proceed with the awarding of the above-described assignment due to the effect of supervening regulatory provisions and/or obstructive circumstances. /p /p #J-18808-Ljbffr

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/7gwlp3