MSCA Doctoral Network PhD position: MS-based proteomic profiling of unmodified and modified β-cateni

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/8cpxrd

Organisation/Company University of Milan Research Field Chemistry » Biochemistry Researcher Profile First Stage Researcher (R1) Positions PhD Positions Application Deadline 31 Oct 2026 - 23:59 (Europe/Vienna) Country Italy Type of Contract Temporary Job Status Full-time Hours Per Week 40 Offer Starting Date 1 Jan 2027 Is the job funded through the EU Research Framework Programme? Horizon Europe - MSCA Marie Curie Grant Agreement Number 101311592 Is the Job related to staff position within a Research Infrastructure? Yes
Offer Description University of Milan
Project Title: MS-based proteomic profiling of unmodified and modified β-catenin- and β-catenin-peptide binder-sequences to decipher the role of protein PTMs in the IDR-mediated β-catenin interactome
Objectives:
- Develop and benchmark quantitative MS-based proteomic strategies to resolve PTM-dependent β-catenin interaction networks, integrating affinity-based interactomics with perturbation-driven proteomic readouts.




- Define how individual and combinatorial PTMs—including S/T phosphorylation and R methylation—rewire β-catenin/IDR interaction networks and propagate into measurable cellular proteomic responses.
- Establish quantitative proteomic signatures linking defined molecular perturbations of the β-catenin system to interaction-network remodeling and downstream pathway states, thereby distinguishing direct PTM-dependent interactions from secondary cellular responses.

Project Overview: Recent evidence shows that PRMT2 directly methylates β-catenin, promoting its proteasomal degradation and thereby attenuating downstream transcriptional activity. This places arginine methylation upstream in the regulation of β-catenin protein stability and provides a compelling mechanistic foundation for investigating β-catenin methylation and its crosstalk with phosphorylation. Building on these findings, this project will investigate how specific PTMs—and defined combinations of PTMs—encode different molecular and cellular

Vedi l’offerta su Go Lavoro: golavoro.it/annuncio-lavoro/8cpxrd